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Snapgene12/4/2023 ![]() ![]() Sticky ends from different EcoO109I sites may not be compatible. Prolonged incubation with NdeI may lead to removal of additional nucleotides.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. ![]() PciI is inhibited by nonionic detergents.īsrGI is typically used at 37☌, but is even more active at 60☌. Sticky ends from different AflIII sites may not be compatible.
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